Spawn & Substrate

Ecovative is the world’s leading mycelium technology company. Using mushroom industry infrastructure, we’ve developed efficiencies and high-quality processes to deliver mycelium products to consumer markets over the last 16 years. With the acquisition of Lambert Spawn Europa, we’ve combined expertise across our organizations to continue to deliver high quality spawn and substrate to current and new mushroom growers as well as our quickly expanding global network of customers growing mycelium materials.

Mushroom Spawn

Quality Grain Spawn for Commercial Mushroom Growers
Exotic Mushroom Substrate

Exotic Substrate

Pre-Inoculated and Incubated Blocks for Optimal Growth

Inoculated Substrate

Fully Colonized Bags of Ecovative’s Signature Substrate Blends

State of the Art Facilities

Ecovative Spawn and Substrate Operations team at works

Leaders in Innovation

  • Same proven strains combined with Ecovative’s improved processes yield better quality products.
  • Experienced team to support your business with know-how and cutting-edge practice.
  • Opportunities for growers to expand their businesses into new markets to open up additional revenue streams.
Ecovative Spawn and Substrate substrate on racks

Efficient Production

  • Automated systems and best practices provide growers with the most consistent spawn available.
  • Mechanical fluid bed technology guarantees exceptional process homogeneity.
  • Unparalleled reproducibility between batches in minimal process time for unmatched efficiency.
Ecovative Spawn and Substrate Mycologist at work in lab

Reliable Practices

  • Every culture selected is approved by our skilled mycologists.
  • Substrates are aseptically filled, then placed in HEPA-filtered rooms designed to enhance stable organism growth.
  • Once fully colonized, all spawn must pass our rigid QA protocols before being transferred into inventory.

Our Facilities

Ecovative Spawn and Substrate operates a state-of-the-art production facility in Venlo, Netherlands. Our skilled team provides the highest quality Lambert spawn to mushroom farms around the world. We’re dedicated to supporting our customers to grow their business.

Our Strains

At Ecovative Spawn and Substrate, expert research, strain development, and technological innovation are the heart of our operations. Our best-in-class laboratories, pilot production facilities, and experienced staff are backed by Ecovative’s 16 years of research into mycelium technology. This means high performing strains, and spawn that excels in terms of yields, quality, and resistance to contamination.

Hybrid Off-whites

Hybrid off-whites perform extremely well in a variety of conditions and are highly suited for fresh markets due to their excellent shelf life. These strains produce high yields of excellent quality with proper handling and environment.
  • Strain 901: Our most popular and bestselling white Agaricus strain. A mid-range hybrid leaning more towards a U1 type. Produces high quality mushrooms with a well-rounded cap suitable for the fresh market. It is a very forgiving strain that responds well to a wide range of cropping and sizing techniques.
  • Strain 920: A more aggressive and very productive strain. Pins slightly easier than our 901.
  • Strain 931: High yielding mushroom with a dense cap and stem. Give easier longer stems which makes it well suitable for mechanical harvesting.


Brown strains are more sensitive and less forgiving than hybrid off-whites. Over-watering and sharp environmental changes should be avoided. Cap color will darken with exposure to light at pinning. Also, drenching with chlorine at a minimum of 150 ppm will help develop mushrooms with a rich, dark color. Good harvesting techniques and pruning are vital for Portabella production.
  • Strain 818: The 818 is a highly productive brown hybrid strain. It gives a nice brown cap with good quality and yield. It’s genetically different to other strains on the market. The required growing style is close to the growing style used for white hybrids. It’s known for strong vegetative growth and relatively easy pin-setting. It’s a very productive strain, especially in the later breaks.

Oyster Spawn

  • Strain 123 Pleurotus ostreatus: Produces a dense fruit with a large, deep gray colored cap. These mushrooms possess a nice shelf-life.
  • Strain 124 Pleurotus ostreatus: Has light gray to white caps and is a very prolific producer that is easy to cultivate.
  • Strain 931: Generates abundant clusters of lemon-yellow caps with bright white gills.
  • Strain 126 Pleurotus pulmonarius: Produces caps that are a rich chocolate brown color. The fruit tends to grow in small clusters.

Shiitake Strains

  • Strain 3000: Strain 3000 produces high quality mushrooms with large dense caps. Tolerant of a variety of substrates and growing conditions although it produces better in cooler temperatures.

Inoculated Substrate

Ecovative is at the forefront of mycelium and mushroom cultivation with our inoculated substrates, developed in the most advanced facilities in the Netherlands and North America. Each batch of our inoculated substrate is a product of meticulous research and rigorous laboratory testing. They are shipped fully colonized and ready to grow, streamlining the cultivation process and maximizing yield potential for our clients.


Ecovative’s original MycoComposite™ substrate blend is made with hemp hurd and our proprietary mycelium strain, ready to produce eco-friendly and strong composites for various products like Mushroom® Packaging or construction materials, location-based licenses may apply.

Colonized Oyster Substrate

Our proprietary Grey Pearl Oyster Substrate arrives fully colonized and ready-to-fruit, crafted from an organic master’s mix. This nutrient-rich blend is designed to yield large, delicious oyster mushrooms quickly. Bags are pre-sterilized to ensure an efficient growing experience.

Looking to learn more or order from Ecovative Spawn and Substrate’s product offerings, fill out our inquiry form.

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